[ebook] Gene Cloning and DNA Analysis 8th Edition An Introduction > Life Sciences

Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.  

Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like: 

• The strategies used by researchers and industry practitioners to assemble genome sequences 
• Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes 
• Includes the use and application of gene editing strategies  
• Interbreeding between Neanderthals and Homo Sapiens  

Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis. 

1. Cover
2. Title Page
3. Copyright Page
4. PREFACE TO THE EIGHTH EDITION
5. PART I: The Basic Principles of Gene Cloning and DNA Analysis
6. Chapter 1: Why Gene Cloning and DNA Analysis are Important
7. 1.1 The early development of genetics
8. 1.2 The advent of gene cloning and the polymerase chain reaction
9. 1.3 What is gene cloning?
10. 1.4 What is PCR?
11. 1.5 Why gene cloning and PCR are so important
12. 1.6 How to find your way through this book
13. FURTHER READING
14. Chapter 2: Vectors for Gene Cloning: Plasmids and Bacteriophages
15. 2.1 Plasmids
16. 2.2 Bacteriophages
17. FURTHER READING
18. Chapter 3: Purification of DNA from Living Cells
19. 3.1 Preparation of total cell DNA
20. 3.2 Preparation of plasmid DNA
21. 3.3 Preparation of bacteriophage DNA
22. FURTHER READING
23. Chapter 4: Manipulation of Purified DNA
24. 4.1 The range of DNA manipulative enzymes
25. 4.2 Enzymes for cutting DNA – restriction endonucleases